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kb dna pieces  (TaKaRa)


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    Structured Review

    TaKaRa kb dna pieces
    Genomic <t>DNA</t> or BAC DNA isolation and purification was followed by size fractionation and ligation into <t>a</t> <t>pUC118</t> ready vector for 4 o C followed with transformation by electroporation into DH5α. The quality of thus constructed shotgun library was checked by titering (40 µl of cell stock, white: blue = 400∶100). The number of clones was approximately 20,000 in total. 96 clones were selected and sequenced including insert size check, E. coli and vector % check.
    Kb Dna Pieces, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+dna+pieces/pmc03877225-202-14-29?v=TaKaRa
    Average 94 stars, based on 25 article reviews
    kb dna pieces - by Bioz Stars, 2026-08
    94/100 stars

    Images

    1) Product Images from "Analysis of the Genome of a Korean Isolate of the Pieris rapae Granulovirus Enabled by Its Separation from Total Host Genomic DNA by Pulse-Field Electrophoresis"

    Article Title: Analysis of the Genome of a Korean Isolate of the Pieris rapae Granulovirus Enabled by Its Separation from Total Host Genomic DNA by Pulse-Field Electrophoresis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0084183

    Genomic DNA or BAC DNA isolation and purification was followed by size fractionation and ligation into a pUC118 ready vector for 4 o C followed with transformation by electroporation into DH5α. The quality of thus constructed shotgun library was checked by titering (40 µl of cell stock, white: blue = 400∶100). The number of clones was approximately 20,000 in total. 96 clones were selected and sequenced including insert size check, E. coli and vector % check.
    Figure Legend Snippet: Genomic DNA or BAC DNA isolation and purification was followed by size fractionation and ligation into a pUC118 ready vector for 4 o C followed with transformation by electroporation into DH5α. The quality of thus constructed shotgun library was checked by titering (40 µl of cell stock, white: blue = 400∶100). The number of clones was approximately 20,000 in total. 96 clones were selected and sequenced including insert size check, E. coli and vector % check.

    Techniques Used: DNA Extraction, Purification, Fractionation, Ligation, Plasmid Preparation, Transformation Assay, Electroporation, Construct, Clone Assay



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    kb dna pieces  (TaKaRa)
    94
    TaKaRa kb dna pieces
    Genomic <t>DNA</t> or BAC DNA isolation and purification was followed by size fractionation and ligation into <t>a</t> <t>pUC118</t> ready vector for 4 o C followed with transformation by electroporation into DH5α. The quality of thus constructed shotgun library was checked by titering (40 µl of cell stock, white: blue = 400∶100). The number of clones was approximately 20,000 in total. 96 clones were selected and sequenced including insert size check, E. coli and vector % check.
    Kb Dna Pieces, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kb+dna+pieces/pmc03877225-202-14-29?v=TaKaRa
    Average 94 stars, based on 1 article reviews
    kb dna pieces - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Genomic DNA or BAC DNA isolation and purification was followed by size fractionation and ligation into a pUC118 ready vector for 4 o C followed with transformation by electroporation into DH5α. The quality of thus constructed shotgun library was checked by titering (40 µl of cell stock, white: blue = 400∶100). The number of clones was approximately 20,000 in total. 96 clones were selected and sequenced including insert size check, E. coli and vector % check.

    Journal: PLoS ONE

    Article Title: Analysis of the Genome of a Korean Isolate of the Pieris rapae Granulovirus Enabled by Its Separation from Total Host Genomic DNA by Pulse-Field Electrophoresis

    doi: 10.1371/journal.pone.0084183

    Figure Lengend Snippet: Genomic DNA or BAC DNA isolation and purification was followed by size fractionation and ligation into a pUC118 ready vector for 4 o C followed with transformation by electroporation into DH5α. The quality of thus constructed shotgun library was checked by titering (40 µl of cell stock, white: blue = 400∶100). The number of clones was approximately 20,000 in total. 96 clones were selected and sequenced including insert size check, E. coli and vector % check.

    Article Snippet: Equivalent volumes of fosmid DNA clones were digested with Not I to obtain 3-7 kb DNA pieces that were then ligated into a purified pUC118 Bam HI/BAP ready vector (Takara Bio Inc., Shiga, Japan) .

    Techniques: DNA Extraction, Purification, Fractionation, Ligation, Plasmid Preparation, Transformation Assay, Electroporation, Construct, Clone Assay